Titration of epithelial suspensions and subsequent analysis with the RT-LAMP-LFD assay.
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A 10% epithelial homogenate (w/v) containing 106 TCID50/mL FMDV was diluted in nuclease free water as described below. These dilutions were each assayed in duplicate with the RT-LAMP-LFD protocol, and analysed with AGE and on an LFD. (a) Application of 5 uL of the “neat” 10% epithelial homogenate added directly to the RT-LAMP-LFD reaction, (b) 1 in 3 dilution, and (c) 1 in 5 dilution, (d) 5 ul of nuclease free water added to the reaction mixture.
创建时间：
2016-02-23
