Identification of glutamate-regulated genes in Arabidopsis seedlings

To understand the global effect of Glu on gene expression, we performed RNA-seq analysis to compare the gene expression profiles between 12-day-old Arabidopsis seedlings grown on 5 mM NH4NO3 and 5 mM Glu as the sole N source. Total RNA extracted from 12-day-old Arabidopsis seedlings grown on medium containing 1/2 MS-modified basal salt mixture without N (M531, PhytoTechnology Laboratories) plus 0.5% sucrose and supplemented with 5 mM NH4NO3 or 5 mM Glu as the sole N source was used for RNAseq analysis.