Heart fibroblasts from Siah-knockout mice in hypoxia. Heart fibroblasts from Siah-knockout mice in hypoxia

Heart fibroblasts from wildtype mice and Siah2-/- knockout mice were isolated and cultured. The cells were either left untreated or incubated for 6 hs under hypoxic conditions. One experiment consists of wildtype cells (normoxia/hypoxia) and Siah2 knockout cells (normoxia/hypoxia) = 4 samples. To allow statistical analysis of the data set the experiment was repeated once under identical conditions. Overall design: 4 groups, each with 2 biological replicates.