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Clostridioides difficile CRISPR-cas9 system

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NIAID Data Ecosystem2026-03-10 收录
下载链接:
https://discovery.biothings.io/dataset/968005dab0e37928
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We developed a CRISPR-cas9 mutagenesis system for use in C. difficile. As a test of the system, we generated a deletion in the pyrE gene of C. difficile R02091. We sequenced to pyrE deletion isolates (to determine if there were off-target effects of the CRISPR system), an FOA-resistant R20291 isolate and our parent R20291 strain for comparison.
创建时间:
2022-06-10
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