Genome-wide identification of transcriptional targets for H4K12la in total brain cells and microglia of WT and 5XFAD mice

In this study, we found that H4K12la level is elevated in plaque-adjacent microglia of 5XFAD mice. To further explore the potential functional significance of H4K12la in AD, we performed genome-wide cleavage under targets and tagmentation (CUT&Tag) analysis to identify candidate genes regulated by H4K12la in total brain cells and microglia. Following CUT&Tag, H4K12la-associated DNAs were amplified using non-biased conditions, labeled, and sequenced with Illumina NovaSeq 150PE. Overall design: Examination of transcriptional targets for H4K12la in total brain cells and microglia of WT and 5XFAD mice.