Determination of the kinetic parameters kon and koff and affinities KD of the GFP:GFP-Nb interaction on different biosensor chip surfaces.
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a The kinetic parameters kon and koff were determined by fitting the SPR data to a 1:1 interaction model (Fig 3).b The KD parameters of the GFP:GFP-Nb interaction were determined from a fitting of the end-point response to a binding isotherm (Fig 4).Determination of the kinetic parameters kon and koff and affinities KD of the GFP:GFP-Nb interaction on different biosensor chip surfaces.
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2015-12-03



