Isolation and functional charaterization of Regulatory T cells from Human peripheral blood

Regulatory T cells are known to supress wide range of anti-tumour responses. It is now evident that Tregs are one among the main barriers for cancer immunotherapy. Until now there have been several methods described to isolate Tregs. It is also been documented that the immunosuppressive functions of CD4+CD25High Tregs in cancer patients is significantly higher than in healthy donors. This study has been designed to isolate CD4+CD25highCD127low/- Tregs from human PBMCs (healthy donors) and functionally characterize them based on their immunosuppression ability