Investigating Genomic Interactions of Werner Syndrome Protein with DamID-seq

We created single clones of HT1080 cells stably expressing low-levels of DamID-alone and DamID-WRN constructs using lentiviral particles. Clone expressing low and comparable levels of DamID-alone and DamID-WRN were used for subsequent experiments. We either mock- or treated with DNA damaging agents, purified genomic DNA from one-three million cells and then specifically amplified m6A-methylated DNA. Subsequently, we processed the PCR amplified DNA for HiFi genome sequencing and obtained raw sequencing data files in the form of fastq files. All sequencing was generated by the UT Southwestern Medical Center, Genomic sequencing Core using an Illumina HiSeq 2500 v3 sequencing system.