ATF1 regulating signaling pathways between nasopharyngeal carcinoma cells and immortalized epithelial cells

The aim of this study is to provide a comprehensive pathways regulated by ATF1 and to screen the differences between cancer and normal cells. Genome-wide mRNA expression microarrays were performed using RNA isolated from ATF1 over-expressing CNE1 and NP69 cells. The ATF1 regulating expression pattern and pathways were analyzed by KEGG pathway, GO annotation and PPI networks. A total of 1651 genes were differentially expressed between CNE1-ATF1 and CNE1-control cells, among which 799 genes were up-regulated and 852 genes were down-regulated. A total of 4304 genes were differentially expressed between NP69-ATF1 and NP69-control cells, among which 2071 genes were up-regulated and 2233 genes were down-regulated. CNE1-ATF1 was designated as the CNE1 cells stably expressing ATF1, and CNE1-control as the negative control. NP69-ATF1 was the NP69 cells stably expressing ATF1, while NP69-control as the negative control. Cells (5×105) were inoculated in a 60 mm culture dish for 2 days, and then RNA was isolated and measure changes in gene expression.