Effect of PPA and BA on neurotransmitter related gene expression in PC12 cells.

PC12 cells were treated with 1 mM PPA, 10 mM PPA, 1 mM BA, 6 mM BA or vehicle for 48 hrs. Total RNA samples were isolated from individual petri dishes (n>6 per experimental group). Northern blot analysis was performed as described in Methods section, using probes specific for rat genes, encoding tyrosine hydroxylase (TH), dopamine beta-hydroxylase (DBH), neuropeptide Y (NPY), Chromogranin A (ChrA), pre-proenkephalin (PENK) and GTP cyclohydrolase (GTPCH). The results are presented as mean ± SEM value relative to the mRNA levels in control, vehicle treated cells. 18S rRNA was used as a house keeping gene for normalization of data. Effect of PPA and BA on neurotransmitter related gene expression in PC12 cells.