Additional file 3: Figure S2. of TRAPLINE: a standardized and automated pipeline for RNA sequencing data analysis, evaluation and annotation

Visualization for RNA transcript quality control and comparison of per base quality score Q. The images are taken before (A) and after (B) quality trimming procedure (removes reads with Q â ¤ 20) to estimate the effect of trimming. The quality score Q is plotted to the read position by using the FastQC package in Galaxy (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/). The color indicates the quality of the read: "red" low quality, "orange" median quality, "green" good quality. Red line expresses the mean of the measured values (yellow boxes are inter-quartile range) and the blue line represents the mean quality. (ZIP 81 kb)