Single Cell RNA-seq data of C1-sgControl, C1-sgEED and C1-sgKMT2D cells. Single Cell RNA-seq data of C1-sgControl, C1-sgEED and C1-sgKMT2D cells

Analysis of gene expression patterns at single cell level of C1-sgControl, C1-sgEED and C1-sgKMT2D cells. Overall design: HMLER-derived C1 cells were gene-edited to generate C1-sgControl, C1-sgEED and C1-sgKMT2D cells. Knock-out of EED or KMT2D enabled the initially stable epithelial C1 cells to gain epithelial-to-mesenchymal plasticity and generated more mesenchymal cell populations.C1-sgControl. C1-sgEED and C1-sgKMT2D cells were stained using anti-human Hashtag antibody associated with three distinct barcodes (BioLegend) before library preparation