Cytotoxicity of different cell lines with bacterial culture supernatant of netF-positive (JFP726, JFP728) and cpb-positive (NCTC3110) C. perfringens strains.
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1EO: Equine ovarian cell line, MDCK: Madin Darby canine kidney cell line, A72: Canine fibroblasts cell line, MDBK: Madin Darby bovine kidney cell line, PK15: Porcine kidney cell line, 208F: Rat Fischer fibroblast cell line, N1H 3T3: Mouse embryo fibroblast cell line, CaCo2: Human colon epithelial cell line, LMH: Primary chicken hepatocellular carcinoma epithelial cell line, Vero: African green monkey kidney cell line.2 Cytotoxicity was evident after 8 h of exposure of 2-fold dilution series of the filter-sterilized culture supernatants up to 1:1024 to different cells. The titer is the final dilution showing > 2+ cell toxicity.3 No cytotoxicity.Cytotoxicity of different cell lines with bacterial culture supernatant of netF-positive (JFP726, JFP728) and cpb-positive (NCTC3110) C. perfringens strains.
