Data for: In-vitro estrogenic activity of binary and multicomponent mixtures with bisphenol A

Bisphenol A and its analogs are well known endocrine disruptors with estrogenic and anti-androgenic activities. In studies of human biomonitoring, simultaneous exposure to multiple bisphenols was shown in different biological samples, at picomolar to low nanomolar concentrations. Evaluation of their combined toxicities will therefore be a more realistic and reliable predictor for estimation of health risks than evaluation of only the single chemicals. In the present study, estrogenic activities of individual bisphenols were evaluated, along with their binary and multicomponent mixtures, using the Organisation for Economic Co-operation and Development validated transactivation assay with the hERα-Hela9903 cell line. Concentration-dependent estrogenic activity was confirmed for all of the tested bisphenols, in the nanomolar to micromolar range. Estrogenic activities of binary and multicomponent mixtures followed a concentration addition model. Although exposure to individual bisphenols remains below their effective doses, we demonstrate that as a mixture, they can contribute additively to toxicity. This study thus emphasizes the importance of mixture toxicity evaluation for risk assessment of compounds that act like the bisphenols.双酚A（Bisphenol A）及其类似物是一类公认的具有雌激素活性与抗雄激素活性的内分泌干扰物。在人体生物监测研究中，不同生物样本中均检测到多种双酚类物质的同时暴露，其浓度范围覆盖皮摩尔至低纳摩尔水平。相较于仅针对单一化学品的毒性评估，对其联合毒性进行评价，是更为贴合实际且可靠的健康风险预测方法。本研究采用经经济合作与发展组织（Organisation for Economic Co-operation and Development，OECD）验证的反式激活试验，以hERα-Hela9903细胞系为测试模型，对单一双酚类物质及其二元、多组分混合物的雌激素活性开展了评估。结果显示，所有受试双酚类物质在纳摩尔至微摩尔浓度范围内均表现出浓度依赖性雌激素活性。二元及多组分混合物的雌激素活性符合浓度加和模型。尽管单一双酚类物质的暴露水平仍低于其有效剂量，但本研究证实，当这些物质以混合物形式存在时，可产生毒性相加作用。本研究由此强调，对于此类具有类双酚作用的化合物，开展混合物毒性评估对其风险评估具有重要意义。