LncRNA NMR knockdown and overexpression in esophageal squamous cell carcinoma cell lines

Long noncoding RNAs (lncRNAs) are emerging as key regulators in cancer and play complicate and critical roles in regulating various key biological processes including chromatin modification, transcription and post-transcriptional processing. We identified a novel transcript, lncRNA NMR, which was upregulated in esophageal squamous cell carcinoma (ESCC) and significantly associated with overall survival of ESCC patients and and is identical to ENST00000432429.1 in GENCODE v13 or ENST00000432429.5 in Ensembl release 83. Here, we sought to determine the alterations of trancriptome after shRNA-mediated knockdown and transient overexpression of lncRNA NMR in ESCC KYSE70 and KYSE450 cell lines． Knockdown and overexpression of LncRNA NMR were performed in ESCC KYSE70 and KYSE450 cell lines with controls, respcectively. A total of 8 samples (2 knockdown with 2 controls and 2 overexpression with 2 controls) were used for RNA sequencing analysis.