Genome-wide Transcriptional Profiling of Nitrogen-Depleted E. coli Treated with Stapled Peptide Inhibitors of the Sigma-54 Promoters

We report the genome-wide effects of blocking the interaction between the sigma-54 bacterial transcriptional enhancer with its cognate promoter in E. coli under nitrogen-starvation conditions. Bacterial mRNA profiles of E. coli grown in nitrogen-deficient media and treated with different sigma-54-based stapled peptides were generated by deep sequencing in triplicate