MLL5 is involved in retinal photoreceptor maturation through facilitating CRX-mediated photoreceptor gene transactivation

Histone methylation, particularly at the H3K4 position, is thought to contribute to the specification of photoreceptor cell fate; however, the mechanisms linking histone methylation with transcription factor transactivation and photoreceptor gene expression have not yet been determined. Here, we investigated how MLL5 affects photoreceptor gene transcription through Immunoprecipitation (IP) , RNA-seq and Western blotting. Attached are the uncropped immunoblotting and gene expression change between Mll5-WT (WT-1, WT-2) and MLL5-KO (KO-1, KO-2) mice retinas.组蛋白甲基化（Histone methylation），尤其是H3K4位点的修饰，被认为参与感光细胞的命运特化过程；然而，将组蛋白甲基化与转录因子反式激活以及感光细胞基因表达相联系的具体分子机制尚未明确。本研究通过免疫沉淀（Immunoprecipitation, IP）、RNA测序（RNA-seq）以及蛋白质免疫印迹（Western blotting）实验技术，探究了MLL5对感光细胞基因转录的调控作用。本研究附有无裁切的免疫印迹结果，以及Mll5野生型（Mll5-WT，WT-1、WT-2）与MLL5基因敲除型（MLL5-KO，KO-1、KO-2）小鼠视网膜组织的基因表达差异数据。