COMBINATION OF NAA AND TDZ FOR In Vitro MULTIPLICATION OF Eugenia involucrata DC

ABSTRACT Eugenia involucrata DC. (Myrtaceae), an economically important forest tree species, is prized for its timber and fruits, and is also an important ornamental plant. This study aimed to evaluate the effect of Thidiazuron (TDZ) and α-Naphthaleneacetic acetic acid (NAA) on in vitro multiplication of nodal segments of E. involucrata. We tested the effect of the absence and presence of NAA (0.5 µM), combined with TDZ at concentrations of 0, 2, 4, 8, 16, or 32 µM, on the in vitro multiplication of E. involucrata. The use of TDZ combined with NAA (0.5 µM) favored the formation of shoots and buds in the explants, especially at 32 µM TDZ concentration. Intermediate concentrations of TDZ also promoted shoot formation but induced hyperhydricity in the explants. It is possible to induce organogenesis leading to the multiplication of E. involucrata nodal segments using TDZ, preferably combined with NAA.