Table 3 in How is the activity of shikimate dehydrogenase from the root of Petroselinum crispum (parsley) regulated and which side reactions are catalyzed?

Table 3 Identification of SDH products by LC-MS analysis. List of products detected after 30 min incubation with (and without) SDH enzyme at 20 ◦ C. DHS, 3-dehydroshikimate; DHQ, 3-dehydroquinate; PCA, protocatechuic acid; SA, shikimic acid; QA, quinate. *the incubation time was 60 min at 20 ◦ C.SDH from P. crispum root (10 μl with specific activity 470 nmol.min -1.mg -1) was incubated in the reaction mixture (280 μl) containing 2 mM substrate (SA, DHS, DHQ, QA, GA, or PCA) and 0.14 mM cofactor (NADP, NADPH, NAD, NADH) in 100 mM Tris-HCl pH 8 (physiological direction) or 100 mM AMP-NaOH pH 9 at 20 ◦ C for 30 min. The reaction was initiated by adding SDH and stopped by adding 10 μl of 99 % (v/v) formic acid. Blank experiments were prepared similarly, albeit adding formic acid before the enzyme. Each determination was done at least 3-times, the average values and S. D. are shown.
reactionproducts [nmol]SA+ NADPDHS: 5.7 ± 0.7PCA: 0.1 ± 0.0*DHS+ NADPHSA: 4.9 ± 0.2GA: 0.5 ± 0.0PCA: 0.7 ± 0.1DHQ: 1.1 ± 0.0DHS+ NADPGA: 0.1 ± 0.0PCA: 0.3 ± 0.2DHS+ NADPGA: 2.1 ± 0.4– without SDHPCA: 2.7 ± 0.6DHQ+ NADPDHS: 17.4 ± 1.4PCA: 4.9 ± 0.4*DHQ+ NADPHDHS: 3.4 ± 0.8SA: 1.4 ± 0.2GA: 1.1 ± 0.5PCA: 1.3 ± 0.2DHQ+ NADHNo products detected.QA+ NADNo products detected.PCA+ NADPHNo products detected.