Evolutionary Insights and Detection of H5N1 2.3.4.4b in Wildlife in Brazil.

The complete hemagglutinin (HA) genome sequence generated in this study was aligned with representative avian influenza virus (AIV) H5N1 sequences from Brazil and Colombia retrieved from the GenBank database, including the first reported South American H5N1 sequence. Phylogenetic and temporal analyses confirmed the placement of the sequence within clade 2.3.4.4b. To ensure transparency, reproducibility, and availability for future comparative, evolutionary, and epidemiological studies, the assembled sequence and its associated metadata (host species, sampling location, and collection date) were deposited in a public sequence repository.本研究获得的完整血凝素（hemagglutinin, HA）基因组序列，与从GenBank数据库中检索得到的巴西、哥伦比亚代表性禽流感病毒（avian influenza virus, AIV）H5N1序列（包含首条公开报道的南美洲H5N1序列）进行了序列比对。系统发育与时间进化分析证实，该序列隶属于进化分支（clade）2.3.4.4b。为保障研究的透明度、可重复性，并为后续比较研究、进化研究及流行病学研究提供可用数据，本研究已将组装完成的序列及其关联元数据（metadata，宿主物种、采样地点及采集日期）提交至公共序列存储库。