Proteomic Dataset: Mass Spectrometric Identification of Membrane Proteins that Interact with PRELP
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MS proteomics raw data files and a Scaffold file for the following research article. Kosuge H, Nakakido M, Nagatoishi S, Fukuda T, Bando Y, Ohnuma SI, Tsumoto K. (January 8, 2021) Proteomic Identification and Validation of Novel Interactions of the Putative Tumor Suppressor PRELP with Membrane Proteins Including IGFI-R and p75NTR. J. Biol. Chem. 10.1016/j.jbc.2021.100278. Epub ahead of print. Research in brief: Proline and arginine-rich end leucine-rich repeat protein (PRELP) is one of the small leucine-rich repeat proteoglycans (SLRPs), known as a multi-functional and multi-specific ligand. We hypothesized that PRELP interacts with various membrane proteins as is the case with other SLRPs, resulting in the modulation of cellular function. To discover membrane proteins that interact with PRELP, we carried out co-immunoprecipitation coupled with mass spectrometry. We prepared membrane fractions from Expi293 cells overexpressing FLAG-tagged PRELP or control cells and analyzed samples precipitated with anti-FLAG antibody by LC-MS/MS analysis. This data set contains the MS proteomics raw data as follows. PRELP_n1 sample: PRELP_130m_qe8.raw PRELP_n2 sample: PRELP_5ul_130m_qe8.raw Control_n1 sample: mock_130m_qe4.raw Control_n2 sample: mock_5ul_130m_qe4.raw This data set also contains the MS proteomics data analyzed by Scaffold software as follows. 190327_28_PRELP_Condensed_2.sf3
本数据集对应下述研究论文的质谱蛋白质组学原始数据文件与Scaffold分析文件。作者为Kosuge H、Nakakido M、Nagatoishi S、Fukuda T、Bando Y、Ohnuma SI、Tsumoto K，其2021年1月8日发表的研究论文为《推定肿瘤抑制因子PRELP与包括IGFI-R及p75NTR在内的膜蛋白的新型相互作用的蛋白质组学鉴定与验证》，刊载于《生物化学杂志》（J. Biol. Chem.），DOI: 10.1016/j.jbc.2021.100278，提前在线出版。 研究概要：富脯氨酸精氨酸末端亮氨酸富集重复蛋白（Proline and arginine-rich end leucine-rich repeat protein, PRELP）属于小型富亮氨酸重复蛋白聚糖（small leucine-rich repeat proteoglycans, SLRPs）家族，是一类兼具多功能与多特异性的配体。研究团队推测，与其他SLRPs类似，PRELP可通过与多种膜蛋白结合调控细胞功能。为筛选与PRELP存在相互作用的膜蛋白，研究采用免疫共沉淀联合质谱分析的实验策略：从过表达FLAG标签PRELP的Expi293细胞及对照细胞中提取膜组分，利用抗FLAG抗体沉淀靶标复合物后，通过液相色谱-串联质谱（Liquid Chromatography-Tandem Mass Spectrometry, LC-MS/MS）完成样品分析。 本数据集包含如下质谱蛋白质组学原始数据： 1. PRELP_n1样本：PRELP_130m_qe8.raw 2. PRELP_n2样本：PRELP_5ul_130m_qe8.raw 3. Control_n1样本：mock_130m_qe4.raw 4. Control_n2样本：mock_5ul_130m_qe4.raw 此外，本数据集还包含经Scaffold软件分析得到的质谱蛋白质组学数据文件：190327_28_PRELP_Condensed_2.sf3
