BIO407 Group3: CLSM images of DFX treated cells

# REMBI metadata ## Study: This data is part of the practical course BIO407 2026 at the University of Zurich, titled 'Advanced Microscopy: From preparation to data and visualization'. The effects of Deferasirox (DFX) on mitochondria morphology were examined using different microscopy techniques. ## Study Component: Job: Confocal microscopyTask: 8 'Imaging Mitochondira in DFX-treated Cells' Using confocal microscopy, images of DFX-treated and untreated cells were acquired. ## Biosample: COS-7 cells (65536) ## Specimen:Four different conditions: -Control 1: No treatment -Control 2: 5h DMSO treatment -Condition 1: 3h 200uM DFX treatment-Condition 2: 5h 200uM DFX treatment Cells were fixed with 4% PFA, 0.05% GTA, 0.5% Saponin in PHEM.Cells were labeled with DAPI, TOMM20-Alexa488, Tubulin-Alexa568, SiR-Actin. ## Image Acquisition: Images were acquired using a "LeicaFALCON": Leica DMI6000B, Model SP8. Acquired channels: Channel 1: DAPI, 405nm excitation, 415nm-497nm emission Channel 2: Alexa488-TOMM20, 488nm excitation, 497nm-548nm emission Channel 3: Alexa568-Tubulin, 561nm excitation, 578nm-637nm emission Channel 4: SiR-actin, 633nm excitation, 652nm-704nm emission Objective: 63x HC PL APO CS2 oil objective, 1.4NA ## Image Data: BIO407_260303_Group3_SP8_65536.lif/T8_63x_DMSO_1BIO407_260303_Group3_SP8_65536.lif/T8_63x_DMSO_2BIO407_260303_Group3_SP8_65536.lif/T8_63x_DMSO_overviewBIO407_260303_Group3_SP8_65536.lif/T8_63x_3h_DFX_1BIO407_260303_Group3_SP8_65536.lif/T8_63x_3h_DFX_2BIO407_260303_Group3_SP8_65536.lif/T8_63x_5h_DFX_1BIO407_260303_Group3_SP8_65536.lif/T8_63x_no_treatment_1BIO407_260303_Group3_SP8_65536.lif/T8_63x_no_treatment_overview