RNA sequencing of stably edited clonal PRPF31+/- hTERT-RPE1 cells

hTERT RPE-1 (ATCC CRL-4000) cell line was edited using CRISPR Cas9 technology to introduce a heterozygous 1bp insert near the intron 5/exon 6 boundary in PRPF31. PRPF31+/- and wild-type cells were sorted into single cell colonies and monoclonal lines established. 3 PRPF31+/- and 3 WT clones were fractionated into cytoplasmic and nuclear extracts and RNA extracted for RNA sequencing for gene and transcript level expression analysis and analysis of differential splicing.