MNase-seq in Arabidopsis thaliana Col and h2a.w mutants

Col and h2a.w mutants (h2a.w6, h2a.w7, h2a.w6/7, h2a.w-2) bud sample were harvested. Nuclei extracted using Honda buffer was fragmented with mirococcal nuclease (MNase, NEB M0247S). The fragmented DNA was purified by phenol/chloroform/isoamyl alcohol (24:24:1) extraction, followed by ethanol precipitation and 2% agarose gel separation and gel extraction of ~145-150 bp DNA. Purified DNA sent to BGI (Hong Kong) for library preparation and sequencing.