The goal of the experiment is to identify genes involved in T-cell receptor (TCR) signaling and T-cell activation in human T cells. The Jurkat E6 cell line, engineered to express dCas9-KRAB and transd

NIAID Data Ecosystem20

Human cortical organoids with engineered microglia-like cells [CROP-seq]

神经退行性疾病模型构建

CRISPRi筛选

Microglia play vital roles in the emergence and preservation of a healthy brain microenvironment with their impaired functions highlighted in neurodevelopmental and neurodegenerative disorders. Howeve

NIAID Data Ecosystem20

Bcor loss perturbs myeloid differentiation and promotes leukaemogenesis [TargetedCRISPRscreen]

白血病发病机制

CRISPRi筛选

Investigating Bcor target genes in BcorΔE9-10KrasG12D leukaemia with a custom CRISPR dropout screen BcorΔE9-10KrasG12D cell line was transduced with a custom CRISPR library and a dropout screen was pe

NIAID Data Ecosystem30

Additional file 2: of CRISPRO: identification of functional protein coding sequences based on genome editing dense mutagenesis

CRISPRi筛选

功能蛋白编码序列鉴定

Table S1, Hit analysis RKO, DLD1 and NCI-H1299 cell lines (XLSX 74 kb)

Figshare2018-10-20 更新20