Effects of doxorubicin on miRNA expression in tumor cell small extracellular vesicles

To examine the changes of miRNA in the small extracellular vesicles of breast cancer cells receiving DOX chemotherapy. Human breast cancer cells (MDA-MB-231 cells) were cultured with exorcism-free serum, and treated with DOX. After 24h, cell supernatant was collected and small extracellular vesicles were extracted by ultra-fast centrifugation method, and miRNA content in small extracellular vesicles was obtained for high-throughput miRNA sequencing. Overall design: miRNA sequencing of 6 samples of small extracellular vesicles from DOX and no drug (control) -treated human breast cancer cells.