Specific activity of mouse MsrB1-Cys and its mutant forms.
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Catalytic activity of purified recombinant proteins was determined using dabsyl-Met-RO as substrate. MsrB1-Cys: Cys mutant of mouse MsrB1, MsrB1-Cys-Xa: Cys mutant of MsrB1 engineered to contain a factor Xa cleavage site inserted in the coding region between Gly75 an Asn76. Cleaved MsrB1-Cys-Xa: MsrB1-Cys-Xa cleaved with factor Xa. ND: not detected.
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2015-12-02



