Characterizing gene expression after expressing genetically encoded tools to change cellular NADH/NAD+ ratio in living cells

To study the overabundance of reducing equivalents, termed reductive stress, specifically for NADH reductive stress, we introduce a soluble transhydrogenase from E. coli (EcSTH) as a novel genetically encoded tool to promote NADH overproduction Comparative gene expression profiling analysis of RNA-seq data for Hela cells expressing LUC, LbNOX, EcSTH, mitoEcSTH