Repair of airway epithelia requires metabolic rewiring towards fatty acid oxidation [I]

Comparative gene expression profiling analysis of RNA-seq data from CD49f+NGFR+, CD49f-NGFR- cell sorted from differentiated MTEC cultures collected at ALI day 10, and MTEC culture taken at ALI t0 Overall design: Primary cells isolated from tracheas by enzymatic treatment were seeded onto 0.4 µm pore size clear polyester membrane coated with a collagen solution. At confluence, media was removed from the upper chamber to establish an air- liquid interface (ALI). Cells were collected at day 10 post air-exposure. Cultures were trypzinazed and sorted into CD49f+NGFR+ and CD49f-NGFR- subsets