mRNA sequencing (RNA-sequencing ) of human β-cell model EndoC-βH1

The aim of the project is the detect changes in global gene expression of protein coding genes after the knockdown of SLC30A8 gene in human beta cell model - EndoC-βH1. For RNA sequencing post 96h siScramble (n=8) or siSLC30A8 (n=8) transfected EndoC-βH1 cells were used and RNA sequencing was performed using Illumina TruSeq-mRNA library on NextSeq 500 system (Illumina) with paired-end reads (2 × 75 base pairs).