Microbiome and physicochemical factors combined analysis reveals the mechanism of non-chlorine oxidizer potassium persulfate improve traditional culture environment

The new fifth generation disinfection, potassium persulphate (PMPS), is well-known in the aquaculture industry and is distinguished by being safe, effective, and environmentally friendly. The prevention of aquaculture diseases and the enhancement of the environment are two benefits of PMPS in practical aquaculture applications. However, it is not clear by which ways PMPS influences ponds, so we carried out this experiment. The Western Aquatic Modern Agricultural Park in Yanting, Mianyang City, was chosen as the study location for this investigation. We not only measured physicochemical factors datas at various sampling time points, but also compared the microbial community after PMPS application by high-throughput sequencing. The measurement results showed that after application 4h, PMPS significantly increased the dissolved oxygen, temperature and redox potential, but significantly decreased the pH, ammonia nitrogen and conductivity. Microbial community analysis revealed that PMPS improved the aquaculture functions and environment by decreasing the abundance of cyanobium_PCC-6307, and increased the abundance of hgcI_clade, CL500_29_marine_group and norank_f_Steroidobacteraceae at around 4 h. Furthermore, as a disinfectant, PMPS only has an inhibitory effects on aeromonas hydrophila, with a rebound with prolonged application. This study is the first to explore the mechanism of PMPS regulation of traditional pools. The findings demonstrate that PMPS as a regulator, may not only directly control physicalchemical variables but also enhance the environment and functionality of the culture pond by changing the microbial community.