Conditioned place preference D1-cre constitutive inhibition

Figure 4C. Mice strain: D1- Cre Viruses- AAVdj-DIO-eGFP #n=7 AAVdj-CAG-DIO-GPE2 (Kir2.1) #n=7 Coordinates:±2.82,1,-3.69 Apparatus: Each place conditioning apparatus consists of an open field enclosed in separate light- and sound-attenuating chambers. General activity and location in the open field was monitored by video recording. The floor of the open field consisted of interchangeable halves made of one of two textures. The combination of floor textures was selected on the basis of calibration studies observing that mice spend an average of about 50% time on each floor type during preference tests. Thus, the apparatus is "unbiased". Specifically, the floors are either of a rough "crushed ice" texture, coupled with walls on which appear black dots vs. a smooth floor texture coupled with walls on which appear vertical black lines. Prior to and following each session the open field and floors was cleaned using a solution of 5% virusolve. Procedures: An unbiased conditioning procedure consists of the following phases: Handling: 30-min sessions. Pre-conditioning bias test: a single 20 min test session is conducted during which mice are allowed to freely explore the open field with half of the arena containing the "crushed ice" floor and dots walls and half of the arena containing the "smooth" floor and straight lines walls. Conditioning: 3 days 2 session/day. Mice are randomly assigned to one of two groups, pairing cocaine (10 mg/kg IP) to either the ‘crushed ice’ or ‘smooth’ contexts, while saline conditioning (10 ml/kg) occurred on the opposite context. On all sessions, mice have access to the entire apparatus with the same floor and walls type on both sides. Post-conditioning bias test: identical to Pre-conditioning. Each CPP experiment was performed on a different group of mice, where Pre-conditioning and Post-conditioning bias test was measured within the same group of mice.图4C。小鼠品系：D1-Cre；病毒载体：AAVdj-DIO-eGFP（n=7）、AAVdj-CAG-DIO-GPE2（Kir2.1，n=7）；注射坐标：±2.82,1,-3.69。 实验装置：每套位置条件化装置均由置于独立隔音隔光舱内的开放活动场地构成，场地内的总活动量与小鼠位置通过录像进行监测。开放场地的底板由两种纹理的可互换半区组成，底板纹理组合经过预校准实验验证：小鼠在偏好测试中平均会在两种底板类型上各花费约50%的时间，因此该装置为“无偏倚”装置。具体而言，一组半区为粗糙“碎冰”纹理底板，搭配带有黑色圆点的侧壁；另一组半区为光滑纹理底板，搭配带有垂直黑色线条的侧壁。每次实验环节前后，均使用5%病毒溶媒溶液对开放场地与底板进行清洁。 实验流程：无偏倚条件化实验包含以下阶段： 1. 适应处理：每次30分钟的适应环节。 2. 预条件化偏好测试：开展单次20分钟的测试环节，小鼠可自由探索开放场地，场地一半区域为“碎冰”底板搭配圆点侧壁，另一半区域为“光滑”底板搭配直线侧壁。 3. 条件化训练：为期3天，每天开展2个训练环节。小鼠被随机分为两组：一组将可卡因（10 mg/kg 腹腔注射）与“碎冰”环境配对，另一组将可卡因与“光滑”环境配对，而生理盐水（10 ml/kg）则在另一侧环境中完成条件化训练。所有训练环节中，小鼠均可自由访问整个装置，且两侧的底板与侧壁类型保持一致。 4. 后条件化偏好测试：流程与预条件化偏好测试完全一致。 每项条件位置偏好（Conditioned Place Preference, CPP）实验均使用独立的小鼠群组，且预条件化与后条件化偏好测试均在同一小鼠群组内完成。