AMPK phosphosite profiling by label-free mass spectrometry reveals a multitude of mTORC1-regulated substrates

The metabolic kinase AMPK regulates transcription in response to nutrient status. AMPK is highly phosphorylated at position Ser184 in the regulatory beta2-subunit, however it's function is unknown. Our preliminary data indicate AMPK dephosphorylated at beta2-Ser184 has elevated nuclear activity and down-regulates apoptotic pathways in response to environmental insult. Here we compare transcriptomes of HEK293T cells expressing either endogenous level beta2 WT or the non-phosphorylatable mutant beta2-Ser182Ala, to gain insight into functional effects of elevated nuclear AMPK activity To investigate alternation in gene expression and cell signalling network between WT and AMPK B2S184A KI of Hek 293T cell using data obtained from RNA-seq from 4 replicates each.