SIRT1 expression effect on migration and invasion of gastric cancer cells

Analysis of BGC-823 gastric cancer cells with SIRT1 overexpression or knockdown. SIRT1, a NAD+-dependent protein deacetylase, exerts inhibitory effects on migration and invasion of gastric cancer. Results provide insight into the role of SIRT1 in the metastasis of gastric cancer. Four groups: Group 1, Stable lentivirus infected BGC-823 cells with the lentiviral expressing vector. Group 2, Stable lentivirus infected BGC-823 cells with the lentiviral expressing vector containing SIRT1 cDNA sequence. Group 3, Stable lentivirus infected BGC-823 cells with the lentiviral vector containing interference control sequence. Group 4, Stable lentivirus infected BGC-823 cells with the lentiviral vector containing interference sequence targeting SIRT1.