Expression data from oral squamous cell carcinomas, 3A and HO1-N-1

Co-culture with human oral squamous cell carcinoma cells, 3A or NEM, or culture with each of their conditioned medium induced many osteoclasts from osteoclast precursor cells, which were generated by a 24-h pretreatment of RANKL or TNF-α. However, HO1-N-1 cells did not induce any osteoclasts. Osteoprotegerin, a decoy RANKL receptor, denosumab, an anti-RANKL antibody drug, and infliximab, an anti-TNF-α antibody drug, did not prevent this tumor-associated osteoclastogenesis. We compared the expression of molecules associated with osteoclastogenesis between 3A and HO1-N-1 cells. 3A and HO1-N-1 cells were cultured at sub-confluent condition, cells were harvested and extracted total RNA. Gene expression microarray analysis using the Human Gene 2.0 ST Array (Affymetrix, Santa Clara, CA) was consigned to Filgen Inc., Nagoya, Japan. We compared the expression of molecules associated with osteoclastogenesis, IL-1β, IL-6, IL-8, PTHrP, RANKL and TNF-α between 3A and HO1-N-1 cells.