Data for: THE ACTIVITY OF AURORA KINASE B IS REQUIRED FOR DENGUE VIRUS RELEASE

Flaviviruses, such as Dengue (DENV), Zika, Yellow Fever, Japanese Encephalitis and West Nile are important pathogens with high morbidity and mortality. The last estimation indicates that ~390 millions of people are infected by DENV per year. The DENV life cycle occur mainly in the cytoplasm of the infected cells and different cytoplasmic, nuclear and mitochondrial proteins participate in viral replication. In this paper we analyzed the participation of Aurora kinase B (AurKB) in the DENV replicative cycle using the specific AurKB inhibitor ZM447439. The kinase inhibition does not alter the viral protein production/secretion or genome replication but impaired the viral yield without altering the percentage of infected cells. Moreover, confocal microscopy analysis of DENV-infected ZM447439-treated cells show a delocalization of viral components from the replicative complexes. In summary, these observations indicate that AurKB participate in DENV viral morphogenesis or release.黄病毒属（Flaviviruses）病毒，包括登革病毒（Dengue virus, DENV）、寨卡病毒、黄热病毒、日本脑炎病毒以及西尼罗河病毒，均为可引发高发病率与高死亡率的重要病原体。最新估算数据显示，每年约有3.9亿人感染登革病毒。登革病毒的生命周期主要发生于受感染细胞的细胞质内，多种胞质蛋白、核蛋白及线粒体蛋白均参与病毒复制过程。本研究通过使用极光激酶B（Aurora kinase B, AurKB）的特异性抑制剂ZM447439，分析了该激酶在登革病毒复制周期中的作用。实验结果表明，抑制该激酶并不会改变病毒蛋白的产生/分泌或基因组复制，但会降低病毒子代产量，且不会影响受感染细胞的比例。此外，对经ZM447439处理并感染登革病毒的细胞开展共聚焦显微镜分析后发现，病毒组分从复制复合物中发生了错位分布。综上，上述观测结果表明，AurKB参与登革病毒的形态发生或释放过程。