Transcriptomic analysis of Omicron Pseudovirus(PsV)-infected HSPCs(hematopoietic stem and progenitor cells)

We have developed a SARS-CoV-2 PsV system that expresses the spike protein on the viral surface and carries spike protein genomic sequences, enabling this pseudovirus to activate infection signaling pathways through ACE2 and stimulate intracellular spike protein gene expression. To understand the impact of SARS-CoV-2 on hematopoietic system, we infected heman HSPCs with Omicron PsV and analyzed changes in transcriptome To investigate the effect of SARS-CoV-2 on hematopoietic system, we constructed pseudovirus system that contained spike genome and expressed spike protein on the surface of virus, which could enter through ACE2.