The model metabolic disruptor DEHP (bis(2-ethylhexyl) phthalate disregulates the gut transcriptome a key player in microbiome-gut axis

Phthalate esters, such as DEHP (bis(2-ethylhexyl) phthalate, are a ubiquitous class of chemicals used as plasticizers in plastic products such as toys and hospital supplies. They are not covalently bound to the polymer matrix and can leach out of products. The goal of this study is to determine whether DEHP adversely affect the zebrafish (Danio rerio) microbiome-gut axis, specifically gut transcriptome. We exposed the fish to 3 ppm DEHP via food for 8 weeks to stimulate chronic exposure. To identify genomic responses of DEHP in gut, we used microarrays followed by differential gene expression analysis. The data were then mapped to changes in cell processes and signaling pathways using Pathway Studio. Zebra fish were exposed to one dose of DEHP (3ppm) : control males (n=5), control females (n=6), DEHP males (n=5), DEHP females (n=7)