Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing [RNA-seq, IL1B treatment]

We analyzed the differential transcriptional response to IL-1ß treatment in cells with experimentally induced hypermethylation of the IL1RN gene promoter. Overall design: We used RNA-seq to investigate how gene expression changes in C/EBPa-B cell-derived macrophages (iMacs) in response to IL-1ß treatment after undergoing CRISPR-dCas9-DNMT3A-induced hypermethylation editing at the IL1RN gene. We compared the gene expression in the edited iMacs (sgIL1RN) to that in non-edited iMacs (CTRL) at three different time points (0 hours, 3 hours, and 16 hours), using biological duplicates for each time point.