Systemin-induced resistance against Botrytis cinerea in tomato plants. UHPLC-MS/MS

In this study, tomato plants were exogenously treated with either 20nM of he peptide systemin at 20nM or water by soil drenched. 24 hours after the treatment, tomato plants were challenged with the necrotrophic fungus Botrytis cinerea. We performed proteomic, metabolomic and enzymatic studies to decipher the Sys-induced changes in tomato plants either in the absence or the presence of Botrytis cinerea infection. Sys-treatments triggered direct proteomic rearrangement mostly involved in carbon metabolism and photosynthesis. However, the final induction of defence proteins required concurrent challenge, triggering priming of pathogen-targeted proteins. the proteomic analysis revealed that Sys conditioned the primary metabolism towards the production of available sugars that could be fuelling the priming of callose deposition in Sys-treated, furthermore PR1 revealed as key element in System,in-induced resistance.