Whole genome sequence data of Mycobacterium tuberculosis and Mycolicibacterium smegmatis mutants of the riboflavin biosynthetic pathway- Part 1

This dataset contains raw whole-genome sequencing data (FASTQ files) for wild-type and riboflavin biosynthetic gene deletion mutants of Mycobacterium tuberculosis and Mycolicibacterium smegmatis. These strains were generated to investigate the source of metabolic antigens recognized by mucosal-associated invariant T (MAIT) cells, which detect intermediates from the riboflavin biosynthetic pathway. To confirm successful gene deletions and rule out off-target effects, whole-genome sequencing was performed for each mutant and its corresponding wild-type strain. This dataset supports the verification of targeted genetic modifications and enables comparative genomic analyses related to MR1 antigen production in mycobacteria., , # Whole genome sequence data of Mycobacterium tuberculosis and Mycolicibacterium smegmatis mutants of the riboflavin biosynthetic pathway- Part 1

Dataset DOI: [10.5061/dryad.4tmpg4fnv](10.5061/dryad.4tmpg4fnv)

## Description of the data and file structure

Whole Genome Sequence of wild type strain and riboflavin biosynthetic pathway mutants of *Mycobacterium tuberculosis* (Mtb) and *Mycolicibacterium smegmatis* (Msm). Also, this dataset has part 2 which is available at https://doi.org/10.5061/dryad.vq83bk454

Naming convention for files: 

1. Lab name 2. Strain Name 3. Sample Number 4. Batch Number 5. Read (Forward/Reverse) 6. Replicate

e.g. For file name \"Dobos_msmegriba2comp_7_S25_L002_R2_001\": Lab name: Dobos; Strain Name: msmegriba2comp_7; Sample Number: S25; Batch Number: L002; Read: R2 (Reverse Read); Replicate: 001

### Files and variables

#### File: Dobos\_mtbribcko\_11\_S29\_L002\_R2\_001.fastq

**Description**: Reverse Read for Mtb *ΔribC*

#### File: Dobos\_msmegriba2comp...,