Single cell RNA-seq of long-term hematopoietic stem cells (LT-HSCs) and common myeloid progenitors (CMPs)

We aimed to reseach the differences between IL4Rα high and low expression LT-HSC/CMP,our data revealed distinct sensitivities of HSCs and common myeloid progenitors (CMPs) to IL4-induced apoptosis, and IL4Rahigh HSCs were more susceptible to unfolded protein response (UPR) and irradiation-induced apoptosis. LT-HSCs and CMPs were purified by FACS sorting based on cell surface markers:Lin-cKit+Sca1+Flt3-CD34- for LT-HSCs and Lin-cKit+Sca1-CD16/32-CD34+ for CMPs.