Changes in the survival rate of cultured cardiomyocytes by an orthogonal experiment.

The results of orthogonal experiments show the effects of 5-HT, GC and NE and their interaction on cell survival. 5-HT>NE >5-HT×GC>GC >5-HT×NE. 5-HT was the most important factor affecting cardiomyocyte apoptosis, followed by NE. The interaction between 5-HT and GC was the third most important factor for cardiomyocyte apoptosis. Within the dose range used, an increase of 5-HT from 10−8 M to 10−6 M promoted cell survival, but the cell survival rate decreased with increases in GC and NE.