Toxicity of LF proteins to RAW 264.7 cells.
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*EC50 is the (effective) concentration of toxin required to kill 50% of cells. RAW264.7 cells were incubated with LF proteins and 250 ng/ml PA and viability determined as described in Methods. #Estimated half life is that measured for a Beta-galactosidase test protein having the same N-terminus when incubated in rabbit reticulocyte lysate or when produced in vivo in Saccharomyces cerevisiae[31]. Half life in Vero-Dr22 cells mentioned here is for diphtheria toxin as reported earlier [32].
创建时间：
2013-02-21
