diabetic cornal immune cells

In this study, we investigated immune cell cluster alterations linked to diabetic corneal diseases using mass cytometry by time of flight (CyTOF). A panel of 41 immune-related markers was used in CyTOF to track changes in the type and quantity of corneal immune cells in the hyperglycemic condition. Comprehensive immunological profiling revealed significant immune cell changes in corneas of diabetic (db/db) mice, including a significant reduction in CD103+CD8+ tissue-resident memory T (TRM) cells and Tregs and a significant increase in γδT cells, a subset of myeloid-derived suppressor cells (MDSCs).本研究采用飞行时间质谱流式细胞术（CyTOF），探究了与糖尿病性角膜疾病相关的免疫细胞集群改变。本研究通过搭载41种免疫相关标志物的CyTOF检测体系，追踪了高糖状态下角膜免疫细胞的类型与数量变化。全面免疫学谱分析显示，糖尿病(db/db)小鼠的角膜组织内免疫细胞发生了显著改变：CD103+CD8+组织驻留记忆T（TRM）细胞与调节性T细胞（Tregs）显著减少，而作为髓系来源抑制细胞（MDSCs）亚群的γδT细胞则显著增多。