PARP activity and effects on primary metabolism.

The metabolite profile of Arabidopsis shoots was analyzed by GC-MS at the indicated times following transfer of the seedlings after 7 days growth on control media to either control or PARP inhibitor containing media. Plants were grown in parallel to those for the physiological and microarray analysis. Five individual samples, each a pool of 10–12 plants, were harvested and analyzed in three independent experiments (n = 15). Amino, organic and fatty acids with significant changes in their relative abundance are shown. Metabolite content relative to the (−3MB), with red and blue indicating accumulation and depletion, are shown.