[³⁵S]GTPγS binding on hH₄R wild-type, hH₄R-F169V and hH₄R-F168A mutant.

pEC50-values ([35S]GTPγS agonist mode), pKb-values ([35S]GTPγS antagonist mode) and α (intrinsic activity, maximal effect relative to histamine = 1.0) are given as mean ± SEM of at least three independent experiments, performed in triplicate. Results of statistical tests (one-way ANOVA and Bonferroni-posthoc-tests): significant differences with respect to hH4R- ● p < 0.05, ●● p < 0.01, ●●● p < 0.001. In case of neutral antagonism (−0.25 ≤ α ≤ 0.25), pKb-values were considered for statistical analysis instead of pEC50-values. Maximal effect α = 0: neutral antagonism. Data for hH4R and hH4R-F169V cf. Wifling et al. [9]. [35S]GTPγS binding on hH4R wild-type, hH4R-F169V and hH4R-F168A mutant.