Saliva microbiota of children with tonsillar and/or adenoid hypertrophy

Participants were required to collect 1.5-2ml of unstimulated saliva after waking up in the morning and a fast of at least 6 hours, before oral hygiene procedures and between 6:00 am and 8:00 am. All participants were asked to sit in a comfortable position in a quiet environment, with their heads naturally tilted downwards, and then were instructed to drool or split saliva directly into the labeled 50 mL collection tube.