Barcode lineage tracking of ferret cortical progeny using TrackerSeq (Mayer lab)

Diversity of cortical radial glia cells (RGCs) and their complex relationships to generate neurons in species with expanded germinal zones and a folded cortex, remains unclear. We used TrackerSeq, a technique that integrates DNA barcodes into the genome of electroporated RGCs, to identify the distinct cell lineages that shape ferret cortex. Overall design: E34 ferret embryos were electroporated with a piggyBac transposon-based library and eGFP+ cells were isolated at E37 by FACS and procesed for their parallel transcriptomic and barcode-lineage tracing characterization.