MICAL1 produces NADP+, H2O2

MICAL1 is involved in cytoskeleton dynamics through its NADPH-dependent oxidase and F-actin depolymerizing activities. MICAL1 inhibits actin polymerization and promotes actin depolymerization (Hung et al. 2011). F-actin is thought to bind and stabilize the flavoprotein domain in MICAL's active conformation (Kolk & Pasterkamp 2007). Actin depolymerization mediated by MICAL1 is thought to be a consequence of H2O2 produced by MICAL1 NADPH oxidase activity (Nadella et al. 2005, Zucchini et al. 2011), rather than due to direct hydroxylation of actin methionine residues (Vitali et al. 2016).

MICAL1通过其NADPH依赖性氧化酶和F-肌动蛋白去聚合活性参与细胞骨架的动态变化。MICAL1能够抑制肌动蛋白聚合并促进肌动蛋白去聚合（Hung等，2011年）。据推测，F-肌动蛋白在MICAL活性构象中与黄素蛋白结构域结合并稳定之（Kolk和Pasterkamp，2007年）。MICAL1介导的肌动蛋白去聚合被认为是MICAL1 NADPH氧化酶活性产生的H2O2的结果，而非直接由于肌动蛋白蛋氨酸残基的羟基化（Nadella等，2005年，Zucchini等，2011年）。